Explanation:

DNA helicase unwinds the double helix, separating the two strands so they may be replicated by DNA polymerase.

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Primase adds an RNA primer to help initiate DNA replication. DNA ligase is responsible for joining Okazaki fragments on the lagging strand during replication.


Explanation:

DNA replication is the process of copying the parent DNA helix into two identical daughter helices. The process is semi-conservative, which means that one parent strand is passed down to each daughter strand. The process begins when helicase unwinds the double helix and separates the two strands to create the replication fork. Topoisomerase helps this process by relieving rotational strain on the helix when it is being unwound. DNA polymerase adds new nucleotides to the daughter strand, synthesizing the new DNA strand.

During replication there is a leading strand, which occurs when replication occurs from 5" to 3" and moves towards the replication fork, and a lagging strand, when replication occurs away from the replication fork. Replication occurs in short segments on the lagging strand, known as Okazaki fragments. The protein DNA ligase is responsible for finally fusing these fragments together after they are made by DNA polymerase.


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Example Question #3 : Dna Replication


During DNA replication, an enzyme called DNA helicase "unzips" the molecule of double-stranded DNA. What is the most likely mechanism of DNA helicase?


Possible Answers:

DNA helicase breaks down the hydrogen bonds between purines and purines


DNA helicase breaks down the hydrogen bonds between the purines and pyrimidines


DNA helicase breaks down the covalent bonds between the purines and pyrimidines


DNA helicase breaks down the covalent bonds between pyrimidines and pyrimidines


Correct answer:

DNA helicase breaks down the hydrogen bonds between the purines and pyrimidines


Explanation:

The question states that DNA helicase "unzips" the two strands of DNA; therefore, this enzyme must be breaking down the bonds between base pairs.

The bonds between base pairs are called hydrogen bonds, which is a noncovalent bond. This means that the DNA helicase is breaking down the hydrogen bonds between base pairs in order to separate the two strands. In DNA, there are two kinds of base pairs: purines and pyrimidines. Recall that adenine and guanine are classified as purines whereas thymine and cytosine are classified as pyrimidines; therefore, a base pairing in DNA always occurs between a purine and a pyrimidine. This means that the DNA helicase is breaking down the hydrogen bonds between purines and pyrimidines.


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Example Question #4 : Dna Replication


Of the following DNA replication proteins, which one links the Okazaki fragments of the lagging strand?


Possible Answers:

Single-strand binding proteins (SSB)


Helicase


DNA ligase


DNA polymerase III


DNA polymerase I


Correct answer:

DNA ligase


Explanation:

DNA ligase is the protein responsible for linking, or ligating, Okazaki fragments together in order to form a single complete DNA strand. This action only necessary on the lagging strand; the leading strand can be made continuously by DNA polymerase since it is able to read away from the replication fork in the 3"-to5" direction. Since the DNA polymerase on the lagging strand must read toward the replication form, it cannot by synthesized continuously.


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Example Question #5 : Dna Replication


DNA is naturally found as a double-helix, but for it to replicated it must first be unwound so that DNA replication proteins can access the two strands. The double-helix structure of DNA is very stable, and after being unwound for DNA replication to occur, the two strands can easily return to the double-helix structure. If the strands re-anneal, proteins necessary for DNA replication cannot enter and begin the process of replication.


Which of the following pairs of DNA replication proteins is responsible for unwinding the DNA double-helix and maintaining the separation of the DNA strands?


Possible Answers:

Single-strand binding protein (SSB) and helicase


DNA polymerase and DNA ligase


DNA ligase and helicase


DNA polymerase and helicase


Helicase and single-strand binding protein (SSB)


Correct answer:

Helicase and single-strand binding protein (SSB)


Explanation:

Helicase is the protein resposible for unwinding the DNA double-helix. Single-strand binding proteins attach to the freshly unwound strands of DNA and ensure that the strands do not re-anneal. Helicase creates the replication fork opening, allowing replication proteins to enter and bind; single-strand binding proteins keep the replication fork open as proteins enter.


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Example Question #1 : Dna Replication


Which of the following causes a DNA fragment to be formed in the 5" to 3" direction?


Possible Answers:

None of the choices


Polarity causes DNA polymerase III can only add nucleotides to the 5" end of a DNA strand


Hydrogen bonds prevent DNA polymerase III from adding nucleotides to a DNA strand


Polarity causes DNA polymerase III can only add nucleotides to the 3" end of a DNA strand.


DNA polymerase III can add nucleotides to any random point on a DNA strand regardless of polarity


Correct answer:

Polarity causes DNA polymerase III can only add nucleotides to the 3" end of a DNA strand.


Explanation:

A DNA fragment will be formed in the 5" to 3" direction because of the polarity of the DNA molecule. Adding nucleotides to the 3" end allows DNA polymerase to use the phosphate molecules as "fuel," and add a new nucleotide to the DNA strand.


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Example Question #1 : Dna Replication


Which protein is responsible for the removal of the RNA primer from the 5" end of a lagging strand of DNA, and replacing it with DNA nucleotides?


Possible Answers:

DNA Polymerase I


None of the choices


DNA Polymerase III


Topoisomerase


Primase


Correct answer:

DNA Polymerase I


Explanation:

DNA Polymerase I removes the primer from the 5" end of a lagging strand, and replaces it with DNA nucleotides. This allows DNA synthesis to begin on the lagging strand. 


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Example Question #8 : Dna Replication


Which of the following RNA molecules is responsible for carrying the code that will be read at the ribosome in order to create a protein? 


Possible Answers:

mRNA


snRNA


rRNA


tRNA


Correct answer:

mRNA


Explanation:

Messenger RNA, or mRNA, is the RNA strand that is transcribed from the gene found on DNA. It is responsible for being read by a ribosome in order to create a protein.

Ribosomal RNA (rRNA) forms a structural component of the ribosomes. Transfer RNA (tRNA) carries amino acid residues and provides an anticodon to add the amino acids to the growing protein at the ribosome. Small nuclear RNA (snRNA) are found in the nucleus and help regulate transcription and maintain telomere length.


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Example Question #9 : Dna Replication


DNA replication is semi-conservative. This means that __________.


Possible Answers:

parts from each original strand will be used as templates when creating the new double helix, resulting in a patchwork combination of both original and newly-synthesized nucleotides


both double strands have a newly created strand and an original template strand


an entirely new synthesized DNA molecule is created, while the original double helix stays together


both double strands have different percentages of nucleotides


Correct answer:

both double strands have a newly created strand and an original template strand


Explanation:

DNA replication involves the separation of the two original DNA strands. Both of these strands are then replicated using DNA polymerase. This results in two DNA double helices, each with a new strand and an original strand.

Consider this example, in which the parent strands are represented by "P" and the daughter strands are represented by "D."

Before replication there are two parent strands: PP

The parent strands are split: P P

Daughter strands are made for each parent strand: PDDP

The fully-replicated double strands separate: PD DP

Each final strand has one parent strand (old DNA) and one daughter strand (new DNA).


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Example Question #1 : Dna Replication


The process of DNA replication is considered semiconservative. DNA is created by using another DNA strand as a template, and building a new complementary strand onto the pre-existing strand. Each new DNA molecule contains one strand from the parent template, and one newly synthesized strand.


A single DNA molecule (one double-helix) undergoes three rounds of replication. After the final replication is complete, how many of the DNA molecules present do not contain any part of the original template?


Possible Answers:

Two


Eight


Four


Six


Zero


Correct answer:

Six


Explanation:

O=Original strand, N=New strand

Before any replications: OO

After one round of replication, both new double-helices contain one strand from the original double-helix and one newly synthesized strand.

1 replication: ON1, N1O

After the second replication, there are now four double-helix molecules. Two contain original strands in combination with new strands, and two contain only new strands.

2 replications: ON2, N2N1, N1N2, N2O

After the thrid replication event there will be eight total molecules. Of these, six will contain only new strands and two will contain a combination of original and new strands.

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3 replications: ON3, N3N2, N2N3, N3N1, N1N3, N3N2, N2N3, N3O

There must always be two double-helices that contain original strands, as there are always only two original strands and they do not disappear.


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