Explanation:

DNA helicase unwinds the dual helix, separating the 2 strands therefore they might be replicated by DNA polymerase.

You are watching: What enzyme unwinds are unzips the parent strand

Primase to add an RNA primer to help initiate DNA replication. DNA ligase is responsible for joining Okazaki fragments on the lagging strand throughout replication.


Explanation:

DNA replication is the procedure of copy the parental DNA helix right into two similar daughter helices. The process is semi-conservative, which way that one parental strand is passed under to every daughter strand. The process begins as soon as helicase unwinds the dual helix and separates the two strands to develop the replication fork. Topoisomerase helps this procedure by relieving rotational strain on the helix once it is gift unwound. DNA polymerase adds new nucleotides to the daughter strand, synthesizing the brand-new DNA strand.

During replication there is a leading strand, i beg your pardon occurs when replication wake up from 5" to 3" and moves in the direction of the replication fork, and a lagging strand, once replication occurs far from the replication fork. Replication wake up in short segments ~ above the lagging strand, recognized as Okazaki fragments. The protein DNA ligase is responsible for ultimately fusing these fragments together after they room made by DNA polymerase.


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Example question #3 : Dna Replication


During DNA replication, one enzyme referred to as DNA helicase "unzips" the molecule that double-stranded DNA. What is the most most likely mechanism of DNA helicase?


Possible Answers:

DNA helicase breaks down the hydrogen bonds between purines and purines


DNA helicase breaks under the hydrogen bonds between the purines and pyrimidines


DNA helicase breaks down the covalent bonds between the purines and pyrimidines


DNA helicase breaks down the covalent bonds between pyrimidines and pyrimidines


Correct answer:

DNA helicase breaks under the hydrogen bonds between the purines and pyrimidines


Explanation:

The inquiry states the DNA helicase "unzips" the two strands the DNA; therefore, this enzyme should be breaking down the bonds in between base pairs.

The bonds in between base pairs are referred to as hydrogen bonds, i beg your pardon is a noncovalent bond. This means that the DNA helicase is breaking under the hydrogen bonds between base pairs in bespeak to different the 2 strands. In DNA, there are two kinds of base pairs: purines and pyrimidines. Recall the adenine and also guanine are classified as purines conversely, thymine and also cytosine room classified as pyrimidines; therefore, a base pairing in DNA constantly occurs between a purine and a pyrimidine. This method that the DNA helicase is breaking under the hydrogen bonds in between purines and also pyrimidines.


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Example question #4 : Dna Replication


Of the following DNA replication proteins, i beg your pardon one links the Okazaki pieces of the lagging strand?


Possible Answers:

Single-strand binding proteins (SSB)


Helicase


DNA ligase


DNA polymerase III


DNA polymerase I


Correct answer:

DNA ligase


Explanation:

DNA ligase is the protein responsible because that linking, or ligating, Okazaki pieces together in bespeak to type a single complete DNA strand. This activity only essential on the lagging strand; the leading strand can be made continuously by DNA polymerase because it is may be to read away from the replication fork in the 3"-to5" direction. Because the DNA polymerase top top the lagging strand have to read toward the replication form, it cannot by synthesized continuously.


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Example inquiry #5 : Dna Replication


DNA is naturally uncovered as a double-helix, however for it come replicated the must very first be unwound so that DNA replication proteins can accessibility the two strands. The double-helix framework of DNA is very stable, and also after gift unwound for DNA replication come occur, the 2 strands can quickly return to the double-helix structure. If the strands re-anneal, proteins vital for DNA replication can not enter and begin the procedure of replication.


Which that the adhering to pairs that DNA replication proteins is responsible for unwinding the DNA double-helix and maintaining the separation that the DNA strands?


Possible Answers:

Single-strand binding protein (SSB) and helicase


DNA polymerase and also DNA ligase


DNA ligase and helicase


DNA polymerase and also helicase


Helicase and also single-strand binding protein (SSB)


Correct answer:

Helicase and single-strand binding protein (SSB)


Explanation:

Helicase is the protein resposible because that unwinding the DNA double-helix. Single-strand binding proteins affix to the freshly unwound strands of DNA and also ensure the the strands perform not re-anneal. Helicase creates the replication fork opening, permitting replication protein to enter and also bind; single-strand binding proteins keep the replication fork open up as proteins enter.


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Example inquiry #1 : Dna Replication


Which of the following causes a DNA fragment to be developed in the 5" come 3" direction?


Possible Answers:

None that the choices


Polarity reasons DNA polymerase III can only add nucleotides to the 5" end of a DNA strand


Hydrogen bonds prevent DNA polymerase III from adding nucleotides to a DNA strand


Polarity causes DNA polymerase III have the right to only add nucleotides to the 3" end of a DNA strand.


DNA polymerase III can add nucleotides to any kind of random allude on a DNA strand regardless of polarity


Correct answer:

Polarity causes DNA polymerase III deserve to only include nucleotides to the 3" end of a DNA strand.


Explanation:

A DNA fragment will certainly be created in the 5" come 3" direction due to the fact that of the polarity the the DNA molecule. Adding nucleotides to the 3" end enables DNA polymerase to usage the phosphate molecules as "fuel," and add a brand-new nucleotide come the DNA strand.


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Example inquiry #1 : Dna Replication


Which protein is responsible because that the remove of the RNA inside wall from the 5" finish of a lagging strand that DNA, and also replacing it with DNA nucleotides?


Possible Answers:

DNA Polymerase I


None that the choices


DNA Polymerase III


Topoisomerase


Primase


Correct answer:

DNA Polymerase I


Explanation:

DNA Polymerase I removes the primer from the 5" end of a lagging strand, and also replaces it through DNA nucleotides. This enables DNA synthetic to begin on the lagging strand. 


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Example inquiry #8 : Dna Replication


Which that the adhering to RNA molecule is responsible for moving the code that will be check out at the ribosom in stimulate to create a protein? 


Possible Answers:

mRNA


snRNA


rRNA


tRNA


Correct answer:

mRNA


Explanation:

Messenger RNA, or mRNA, is the RNA strand that is transcribed from the gene discovered on DNA. That is responsible because that being check out by a ribosomes in order to produce a protein.

Ribosomal RNA (rRNA) creates a structural ingredient of the ribosomes. Carry RNA (tRNA) dead amino mountain residues and provides one anticodon to add the amino acids to the cultivation protein at the ribosome. Small nuclear RNA (snRNA) are discovered in the nucleus and aid regulate transcription and also maintain telomere length.


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Example inquiry #9 : Dna Replication


DNA replication is semi-conservative. This means that __________.


Possible Answers:

parts native each initial strand will certainly be supplied as templates when developing the new dual helix, bring about a patchwork mix of both original and newly-synthesized nucleotides


both dual strands have a newly produced strand and also an original template strand


an entirely brand-new synthesized DNA molecule is created, when the original dual helix stays together


both dual strands have various percentages that nucleotides


Correct answer:

both twin strands have actually a newly developed strand and an original template strand


Explanation:

DNA replication involves the separation of the two original DNA strands. Both of these strands space then replicated using DNA polymerase. This outcomes in 2 DNA dual helices, each with a brand-new strand and also an original strand.

Consider this example, in i beg your pardon the parental strands are represented by "P" and also the daughter strands are stood for by "D."

Before replication there are two parent strands: PP

The parent strands space split: p P

Daughter strands are created each parental strand: PDDP

The fully-replicated twin strands separate: PD DP

Each final strand has actually one parent strand (old DNA) and one daughter strand (new DNA).


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Example concern #1 : Dna Replication


The procedure of DNA replication is taken into consideration semiconservative. DNA is created by using an additional DNA strand as a template, and also building a brand-new complementary strand top top the pre-existing strand. Each brand-new DNA molecule includes one strand indigenous the parental template, and also one newly synthesized strand.


A solitary DNA molecule (one double-helix) undergoes 3 rounds the replication. After ~ the final replication is complete, how many of the DNA molecules current do not contain any component of the initial template?


Possible Answers:

Two


Eight


Four


Six


Zero


Correct answer:

Six


Explanation:

O=Original strand, N=New strand

Before any kind of replications: OO

After one round of replication, both brand-new double-helices contain one strand indigenous the original double-helix and one freshly synthesized strand.

1 replication: ON1, N1O

After the second replication, there space now four double-helix molecules. 2 contain original strands in mix with new strands, and two save only new strands.

2 replications: ON2, N2N1, N1N2, N2O

After the thrid replication event there will be eight full molecules. That these, six will contain only brand-new strands and also two will contain a mix of original and new strands.

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3 replications: ON3, N3N2, N2N3, N3N1, N1N3, N3N2, N2N3, N3O

There must always be 2 double-helices that contain original strands, together there are always only two initial strands and they execute not disappear.


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